In vitro fertilization in the lab by intracytoplasmic sperm injection to subsequently transfer the embryo/s obtained into patient's own uterus expecting it will implant and give rise to a pregnancy.
Women between 18-52 years of age in good physical and mental condition.
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After a period of ovarian stimulation and through follicular aspiration, the oocytes are retrieved from the woman's ovaries. This process is carried out under ultrasound monitoring by the gynecologist who determines the best time, just before ovulation.
In a coordinated manner the man will be asked to provide a sperm sample by masturbating into a specimen cup usually on the same day the treatment takes place. The sperm sample is processed in order to increase the vigor of the spermatozoa through centrigugation and a technique named swim-up.
Good-quality motile spermatozoon are selected one by one. Afterwards it's directly injected into each egg after immobilization through microinjection (ICSI). Intracytoplasmic sperm injection (ICSI) is carried out in the laboratory and subsequent incubation and monitoring are performed to check that the egg/s have been fertilized correctly, producing developing embryos.
One or more embryos are transferred to woman's uterus through an ambulatory painless procedure that does not require sedation, after which rest is recommended.The gynecologist introduces the embryo using a soft catheter under ultrasound guidance.
From 10-12 days after the embryo transfer, a blood test can be performed to identify the presence of the Beta-CGH subunit, confirming the diagnosis of pregnancy.
If there are any oocytes that have been retrieved but not been used for the process, they can be preserved using vitrification if desired. Vitrification procedure allows oocyte preservation and long term storage in custody.
If there are any surplus embryos they can be cryopreserved for own use or donation, under a strict legal protocol that all clinics are required to obey. Embryo cryopreservation is performed by vitrification and they remain in custody.